The miniseries focuses on the three new lead characters of the film Death Note: Light Up the NEW World: Tsukuru Mishima, Ryuzaki, and Yuki Shien. Each episode provides backstory for one of the characters and bridges the 10-year gap between the previous films, which canonically took place in 2005-2006, and the new film taking place in 2016.

The first episode, "Rebirth," focuses on Tsukuru Mishima and how he came to join the Death Note Countermeasure Task Force, which continues to operate to look into deaths that might be caused by a Death Note. The title "Rebirth" is a reference to Kira's return.

The second episode, "Dying Wish," focuses on L's successor Ryuzaki. The story involves him looking into a case regarding a locked-room murder on request of J, another member of Wammy's House. The new film character Sakura Aoi also appears in this episode.

The third episode, "Fanaticism," focuses on Yuki Shien. The story involves the trauma he carries from the murder of his family and why he became a cyber-terrorist following in Kira's footsteps.